Reaction mechanism of nitric oxide reductase and its relation to molecular structure
نویسنده
چکیده
Fungal nitric oxide reductase (cytochrome P450 nor) is a heme-containing enzyme, in which the iron-porphyrin complex is an active site of the enzyme. This enzyme is involved in the fungal denitrification process, and catalyzes the reduction of NO to N2O. Its structural and functional characteristics were examined by enzymatic, kinetic, spectroscopic (optical absorption, ESR, resonance Raman, IR) and crystallographic techniques. On the basis of these results, the molecular mechanism of the NO reduction by P450 nor was proposed.
منابع مشابه
Improvement of Tissue Survival of Skin Flaps by 5α-Reductase Inhibitors: Possible Involvement of Nitric Oxide and Inducible Nitric Oxide Synthase
Background: Skin flap grafting is a popular approach for reconstruction of critical skin and underlying soft tissue injuries. In a previous study, we demonstrated the beneficial effects of two 5α-reductase inhibitors, azelaic acid and finasteride, on tissue survival in a rat model of skin flap grafting. In the current study, we investigated the involvement of nitric oxide and inducible nitric o...
متن کاملMolecular Dynamics Simulation of Al/NiO Thermite Reaction Using Reactive Force Field (ReaxFF)
In this work, the thermal reaction of aluminum (Al) and nickel oxide (NiO) was investigated by molecular dynamics simulations. Some effective features of reaction such as reaction temperature, the reaction mechanism, and diffusion rate of oxygen into aluminum structure were studied. ReaxFF force field was performed to study the Al/NiO thermite reaction behavior at five different temperatures (5...
متن کاملAGE proteins as a causative factor in Alzheimer's Disease
The reaction between reducing sugars and protein free amines, known as the Maillar reaction results in the formation of advanced glycation endproducts (AGEs). AGE modification changes the structure of proteins to amyloid cross-beta structure. These protein structures can activate receptors known as RAGE on glial cells (microglia and astrocytes), and induce the expression of inducible nitric oxi...
متن کاملAGE proteins as a causative factor in Alzheimer's Disease
The reaction between reducing sugars and protein free amines, known as the Maillar reaction results in the formation of advanced glycation endproducts (AGEs). AGE modification changes the structure of proteins to amyloid cross-beta structure. These protein structures can activate receptors known as RAGE on glial cells (microglia and astrocytes), and induce the expression of inducible nitric oxi...
متن کاملBio-scale Engine: Reaction Mechanism of Nitric Oxide Reductase Cytochrome P450nor from Fusarium oxysporum
A unique enzyme mechanism named a bio-scale engine was proposed for a nitric oxide reductase, cytochrome P450nor (P450nor). P450nor has some structural similarities with a combustion engine: the heme pocket, the heme and NADH correspond to a combustion chamber, a piston head, and a spark plug, respectively. Each putative step of this reaction occurred in the heme pocket was characterized using ...
متن کامل